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Decoding histone post-translational modifications by bottom-up mass spectrometry
【2015.1.21 3:00pm, S813】

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 2015-1-20 

  Colloquia & Seminars 

  Speaker

Dr.Zuofei Yuan,Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania  

  Title

Decoding histone post-translational modifications by bottom-up mass spectrometry    

  Time

2015.1.21 15:00-16:00

  Venue

思源楼813

  Abstract

Histone post-translational modifications (PTMs) contribute to chromatin function through their chemical properties which influence chromatin structure, and their ability to recruit chromatin interacting proteins. Nanoflow liquid chromatography coupled with high resolution tandem mass spectrometry (nanoLC-MS/MS) has emerged as the most suitable technology for global histone modification analysis due to the high sensitivity and the high mass accuracy that provide confident identification. However, the histone nanoLC-MS/MS data analysis is even more challenging due to the large number and variety of isobaric histone peptides, and the high dynamic range of histone peptide abundances. Therefore, quantification of histone PTMs has become a high priority to investigate cell regulation and epigenetics. In this study, we developed a program named EpiProfile to solve the problems of histone peptide quantification.    

  Affiliation

 

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